ORF library screen for melarsoprol resistance in T. brucei

T. brucei parasites (African trypanosomes) were transfected with a Dox inducible gain of function ORF library and subjected to treatment with the anti-trypanosomal agent melarsoprol. In order to identify genes whose overexpression can contribute to survival in the presence of melarsoprol, sequence reads were generated from untreated parasites and for those that survived treatment with the drug.

布氏锥虫（Trypanosoma brucei，亦称非洲锥虫）被转染携带多西环素（Dox）诱导型功能获得型开放阅读框（ORF）文库，随后接受抗锥虫药剂美拉胂醇（melarsoprol）处理。为筛选出过表达可提升受试样本在美拉胂醇存在条件下存活率的基因，研究人员分别从未经药物处理的锥虫样本，以及经药物处理后存活的锥虫样本中生成测序读段（sequence reads）。