Analysis of global transcriptional response of Mycobacterium tuberculosis H37Rv to the natural compound ethyl p-methoxycinnamate. Mycobacterium tuberculosis H37Rv

Gene expression profiling analysis was performed after exposing M. tuberculosis H37Rv to EPMC for 24 and 48 hours. Significant changes in expression levels were observed in the genome in response to the drug. Analysis of the data carried out by studying the expression pattern at the level of pathways, indicated that several pathways including amino sugar and nucleotide sugar metabolism, linoleic acid metabolism, fructose and mannose metabolism, galactose metabolism, lysine degradation pathways were significantly upregulated upon exposure to EPMC, while the pathways involved primarily in the mycobacterial cell wall biosynthesis - the mycolic acid biosynthesis and D-alanine metabolism were significantly downregulated Overall design: M. tuberculosis H37Rv cells were grown to log-phase and then treated with the compound ethyl p-methoxycinnamate. The drug isoniazid was taken as apositive control. The cells were exposed to the compounds for 24 and 48 hours. Cells to which no compound was added acted as untreated control. After the time points, the cells were harvested and RNA was isolated. The RNA was subjetced to microarray. The data was analysed at pathway level and gene ontology level.

将结核分枝杆菌（Mycobacterium tuberculosis）H37Rv暴露于对甲氧基肉桂酸乙酯（ethyl p-methoxycinnamate，EPMC）中24小时与48小时后，开展基因表达谱分析。结果显示，该药物作用后基因组的表达水平发生显著变化。通过在通路层面分析表达模式进行的数据解析结果表明，暴露于EPMC后，氨基糖与核苷酸糖代谢、亚油酸代谢、果糖与甘露糖代谢、半乳糖代谢及赖氨酸降解等多条通路均显著上调；而主要参与分枝杆菌细胞壁生物合成的通路——分枝菌酸生物合成与D-丙氨酸代谢通路则显著下调。实验整体设计如下：将结核分枝杆菌H37Rv培养至对数生长期，随后用对甲氧基肉桂酸乙酯进行处理。以异烟肼作为阳性对照。将细胞暴露于该化合物环境中24小时与48小时，未添加任何化合物的细胞作为空白对照。到达预设时间节点后，收集细胞并分离总RNA，将RNA样本进行基因芯片检测。后续分别从通路层面与基因本体（Gene Ontology，GO）层面开展数据分析。