Data Sheet 11_Potential prognostic biomarkers in restenosis of peripheral arteries identified through comprehensive ceRNA network analysis.csv

Peripheral artery disease (PAD) is one of the leading causes of amputation and mortality worldwide, with restenosis being a common complication following vascular interventional therapy that significantly impacts patient prognosis. This study aims to identify potential biomarkers with clinical and prognostic significance for restenosis of peripheral arteries (RPA). To achieve this, RNA expression levels in stenotic vascular tissues from healthy controls and patients with RPA were integrated and analyzed, identifying a growing number of differentially expressed RNAs for the construction of a competitive endogenous RNA (ceRNA) network for prognostic analysis. The analysis revealed that the RNA expression patterns of samples from patients with RPA were significantly different compared with those of healthy controls. Consequently, the resulting differentially expressed RNAs were used to construct a ceRNA network. Through bioinformatics analysis, a complex regulatory network composed of multiple ceRNA axes was established. The findings indicate that key mRNAs in the network play critical roles in various biological functions. Given the prognostic significance of these six differentially expressed genes in RPA, real-time quantitative fluorescence PCR (qRT-PCR) analysis was performed. These results suggest that these candidate key differentially expressed genes are potential prognostic biomarkers for RPA, and their corresponding mRNAs may serve as potential therapeutic targets.

外周动脉疾病（Peripheral artery disease, PAD）是全球范围内导致截肢与死亡的主要病因之一，而再狭窄是血管介入治疗后常见的并发症，会显著影响患者预后。本研究旨在识别具备临床与预后价值的外周动脉再狭窄（restenosis of peripheral arteries, RPA）潜在生物标志物。为达成研究目标，本团队整合分析了健康对照与外周动脉再狭窄患者的狭窄血管组织RNA表达水平，鉴定出大量差异表达RNA，用于构建竞争性内源RNA（competitive endogenous RNA, ceRNA）网络以开展预后分析。分析结果显示，外周动脉再狭窄患者样本的RNA表达模式与健康对照样本存在显著差异。据此，研究人员利用所得差异表达RNA构建了竞争性内源RNA网络。通过生物信息学分析，本研究搭建了由多条ceRNA轴组成的复杂调控网络。研究结果表明，该网络中的关键mRNA在多种生物学功能中发挥关键作用。鉴于这6个差异表达基因对外周动脉再狭窄具有预后价值，本研究开展了实时定量荧光PCR（real-time quantitative fluorescence PCR, qRT-PCR）分析。上述结果提示，这些候选关键差异表达基因可作为外周动脉再狭窄的潜在预后生物标志物，其对应的mRNA或可成为潜在治疗靶点。