Thermodynamic parameters and their associated error from the global fit of DSC and FLT data.

Footnote for Table 3: Note that * indicates parameters obtained from fitting just FLT data sets and † indicates parameters obtained from fitting just DSC data sets. EGTA stands for ethylene glycol tetraacetic acid. NA stands for not applicable and is used for instances where just FLT data was fit. As an additional control, FLT signal was collected at 345 nm, normalized, and subsequently globally fit with DSC denaturation data. The thermodynamic parameters that resulted are similar to those presented above, indicating a lack of water fluorescence contribution at the 340 nm wavelength used for this analysis (Table S3).

表3脚注：请注意，星号*代表仅通过FLT数据集拟合得到的参数，剑号†代表仅通过差示扫描量热法（Differential Scanning Calorimetry，简称DSC）数据集拟合得到的参数。乙二醇双(2-氨基乙基醚)四乙酸（ethylene glycol tetraacetic acid，简称EGTA）。NA（not applicable，不适用）用于仅拟合FLT数据的场景。作为额外对照实验，我们在345 nm波长处采集FLT信号，经归一化处理后与DSC变性数据进行全局拟合。所得热力学参数与前文呈现的结果相近，表明本分析所用的340 nm波长处无水体荧光贡献（详见表S3）。