Study of the influence of the presence of Dr fimbriae on the adherence of cells of the recombinant Escherichia coli strain: BL21(DE3)/pCC90 to glass in a dynamic system

The attachment of bacteria begins the process of surface colonization, called biofilm development, characterized by a number of physicochemical and molecular interactions. Adherence to inert surfaces typically involves nonspecific interactions, whereas adherence to biological surfaces is associated with specific ligand-receptor interactions. In this study, the adherence ability of four genetically modified Escherichia coli strains—BL21(DE3)/pACYCpBAD, BL21(DE3)/pCC90 Dra, AAEC191A/pCC90, and AAEC191A/pACYCpBAD—was examined on both glass and polystyrene surfaces. The aim was to investigate the effect of the presence of Dr fimbriae on the adherence of bacteria to various carriers. The experiments were performed under various flow conditions.
The catalog contains original video data that can be played using Olympus' CellSense software. The videos contain full layers of data characterizing the image recording process.

细菌的附着标志着表面定殖过程的启动，该过程被称为生物膜形成（biofilm development），其特征在于一系列物理化学及分子相互作用。细菌对惰性表面的黏附通常涉及非特异性相互作用，而对生物表面的黏附则与特异性配体-受体相互作用（ligand-receptor interactions）相关。本研究检测了四株基因修饰的大肠杆菌（Escherichia coli）菌株——BL21(DE3)/pACYCpBAD、BL21(DE3)/pCC90 Dra、AAEC191A/pCC90及AAEC191A/pACYCpBAD——在玻璃和聚苯乙烯表面的黏附能力，旨在探究Dr菌毛（Dr fimbriae）的存在对细菌黏附于不同载体的影响。

本目录包含可通过奥林巴斯CellSense软件播放的原始视频数据，这些视频涵盖了表征图像记录过程的完整数据层。