SH1916304.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE 提供了一套统一的解决方案，用于对基于DNA的物种假说（Species Hypotheses，下文简称SH）进行界定、识别、交流与标准化处理。国际核苷酸序列数据库中的全部真菌ITS序列，均通过应用一组动态距离阈值（0.5% - 3.0%），被聚类至近似物种水平的集群单元。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）形式存在的唯一且稳定的名称，其分类学与生态学注释信息则通过分布式的网络第三方注释工作完成验证。系统会结合某一SH下所有序列的鉴定结果，尽可能将该SH与对应的类群名称及其所属的分类层级（门、纲、目等）进行关联。研究人员会选取一条经自动或人工选定的代表序列，用以表征每个对应的SH。上述代表序列已通过（https://unite.ut.ee/repository.php）对外发布，可供科学界用于本地序列相似性搜索、高通量测序分析流程等场景。随着公共真菌ITS序列库规模的不断增长，该系统及其配套数据会自动完成更新。