Small RNA sequencing of oxidative low-density lipoprotein treated endothelial cells

Atherosclerosis (AS) is a common cardiovascular disease with high incidence rate and mortality. The injury and dysfunction of endothelial cells are the early markers of AS. Oxidative low-density lipoprotein (Ox-LDL) is a key risk factor for the development of AS. Ox-LDL promotes endothelial cells apoptosis, induces inflammation and oxidative stress of endothelial cells. Small noncoding RNA (sncRNA) mainly include Piwi-interacting RNA (piRNA), small nucleolar RNA (snoRNA), small nuclear RNA (snRNA), microRNA (miRNA) and repeat-associated RNA. We used small RNA sequencing to analyze the effect of ox-LDL on the expression of sncRNAs in endothelial cells, and identify the sncRNA which is related to ox-LDL induced endothelial cells dysfunction.

动脉粥样硬化（Atherosclerosis, AS）是一种高发且高致死率的常见心血管疾病。内皮细胞损伤与功能异常是动脉粥样硬化的早期标志物。氧化型低密度脂蛋白（Oxidized low-density lipoprotein, Ox-LDL）是动脉粥样硬化发生发展的关键危险因素，其可促进内皮细胞凋亡，诱发内皮细胞炎症反应与氧化应激。小型非编码RNA（Small noncoding RNA, sncRNA）主要包括Piwi相互作用RNA（Piwi-interacting RNA, piRNA）、核仁小RNA（small nucleolar RNA, snoRNA）、核内小RNA（small nuclear RNA, snRNA）、微小RNA（microRNA, miRNA）以及重复序列相关RNA。本研究采用小RNA测序技术，分析氧化型低密度脂蛋白对内皮细胞中小非编码RNA表达的影响，并筛选出与氧化型低密度脂蛋白诱导的内皮细胞功能异常相关的小型非编码RNA。