MOESM5 of ATM controls DNA repair and mitochondria transfer between neighboring cells

Additional file 5: Figure S5. a–f Mitochondria transfer in ATMwt and ATM−/− fibroblasts upon irradiation. Mitochondria transfer was monitored between donor cells labeled with MitoTracker Deep Red (green, indicated with white marker) and 6–Gy irradiated acceptor cells labeled with MitoTracker Red (red, indicated with orange marker) after 24 h of co–culture. Nuclei were stained with DAPI. Co–culture of ATMwt and irradiated ATMwt fibroblasts (a and b), ATMwt and irradiated ATM−/− fibroblasts (c and d), ATM−/− and irradiated ATMwt fibroblasts (e), as well ATM−/− and irradiated ATM−/− fibroblasts (f). g Unilateral transfer of mitochondria from irradiated ATMwt (labeled with MitoTracker Deep Red) to ATM−/− fibroblasts (labeled with MitoTracker Green, indicated with white marker). h Unilateral transfer of mitochondria from ATMwt (labeled with MitoTracker Deep Red, indicated with white marker) to irradiated ATM−/− fibroblasts (labeled with MitoTracker Red). SRRF: super–resolution radial fluctuation images. Scale bars, 10 μm.

附加文件5：图S5。a~f 展示了辐照处理后ATM野生型（ATMwt）与ATM基因敲除（ATM−/−）成纤维细胞的线粒体转移情况。将标记有MitoTracker Deep Red（呈绿色，以白色标记标示）的供体细胞，与经6 Gy辐照、标记有MitoTracker Red（呈红色，以橙色标记标示）的受体细胞进行24小时共培养，随后监测线粒体的转移过程。细胞核采用DAPI染色。本次实验的共培养组合包括：ATMwt与辐照后的ATMwt成纤维细胞（a、b）、ATMwt与辐照后的ATM−/−成纤维细胞（c、d）、ATM−/−与辐照后的ATMwt成纤维细胞（e），以及ATM−/−与辐照后的ATM−/−成纤维细胞（f）。g 为线粒体从辐照后的ATMwt细胞（标记有MitoTracker Deep Red）单向转移至ATM−/−成纤维细胞（标记有MitoTracker Green，以白色标记标示）的成像结果。h 为线粒体从ATMwt细胞（标记有MitoTracker Deep Red，以白色标记标示）单向转移至辐照后的ATM−/−成纤维细胞（标记有MitoTracker Red）的成像结果。SRRF：超分辨率径向波动成像（super-resolution radial fluctuation）图像。图像比例尺为10 μm。