Disulfiram/Cu induce ferroptosis in triple-negative breast cancer cells

To further explore the mechanism of DSF/Cu in TNBC, we conducted transcriptome sequencing experiments in MDA-MB-231 cells in response to 24 h treatment with DSF/Cu (0.2 µM). RNA-seq data revealed that 874 genes were upregulated and 764 genes were downregulated in DSF/Cu treated cells. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that TNBC cells death induced by DSF/Cu wais related to MAPK signaling pathway, p53 signaling pathway, mitophagy signaling pathway and ferroptosis. Overall design: MDA-MB-231 cells were seeded in 6-well plates and incubated with culture mediums. The next day, cells were treated with DMSO or DSF/Cu and incubated for 24 h. The cells of three wells were maxed into one repeat, and three repeats were performed for RNA-sequencing.

为进一步探究DSF/Cu在三阴性乳腺癌（TNBC）中的作用机制，我们对MDA-MB-231细胞开展转录组测序实验，以分析该细胞经0.2 μM DSF/Cu处理24小时后的转录组表达变化。RNA测序（RNA-seq）结果显示，经DSF/Cu处理的细胞中共有874个基因上调、764个基因下调。京都基因与基因组百科全书（KEGG）通路富集分析结果表明，DSF/Cu诱导的TNBC细胞死亡与丝裂原活化蛋白激酶（MAPK）信号通路、p53信号通路、线粒体自噬信号通路以及铁死亡密切相关。实验整体设计如下：将MDA-MB-231细胞接种于6孔板中，置于培养基中培养。次日，分别用二甲基亚砜（DMSO）或DSF/Cu处理细胞，继续培养24小时。每3个孔的细胞合并为一个生物学重复，本实验共设置3个生物学重复用于RNA测序。