Chemical reprogramming of human blood cells into hCiPS cells [bulk ATAC-seq]

Chemical reprogramming offers a fundamentally innovative approach for generating human pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells. Total of 8 bulk ATACseq data were analyzed, which include PBMC EPC, S1, S1-BI-7273, PBMC hCiPSC and 2 biological replicates for each condition.

小分子化学重编程为利用小分子化合物制备人类化学诱导多能干细胞（hCiPS）提供了具有根本性创新意义的技术路径。我们此前的研究表明，该方法可高效将人类成纤维细胞重编程为hCiPS细胞。本研究中，我们建立了一套稳定可靠的实验方法，可成功从脐带血与成人外周血细胞中制备得到hCiPS细胞。该方法对新鲜及冻存的血细胞均能实现高效重编程。本研究对总计8组批量ATAC测序（bulk ATAC-seq）数据进行了分析，样本涵盖PBMC EPC、S1、S1-BI-7273、PBMC hCiPSC，且每组实验条件均设置2次生物学重复。