Determination of MHV virion genome poly(A) tail length and 3' end additions.

The mouse hepatitis virus (MHV) genomic RNA has a poly(A) tail required for replication. Here we investigated the presence of terminal poly(A) tail uridylation and guanylation in the virion RNA. After isolating the viral RNA followed by sequencing, we found a mean poly(A) tail of 66 nucleotides long with a peak of terminal uridylation on tails 55 to 66 nucleotides long. Overall design: Direct RNAseq of RNA isolated from MHV virion

鼠肝炎病毒（mouse hepatitis virus, MHV）的基因组RNA具有复制所必需的poly(A)尾结构。本研究针对病毒粒子RNA中末端poly(A)尾的尿苷酸化与鸟苷酸化现象展开了探究。在分离病毒RNA并进行测序后，我们发现其poly(A)尾的平均长度为66个核苷酸，且在长度为55至66个核苷酸的尾段中，末端尿苷酸化程度达到峰值。实验整体设计：对从MHV病毒粒子中分离得到的RNA开展直接RNA测序（Direct RNAseq）。