Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasis

Analysis of miRNA and mRNA within extracellular vesicles obtained from prostate cancer PC3 cells. Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482 Overall design: miRNAs and mRNA from PC3 extracellular vesicles tested by RNAseq in duplicate using an Illumina Nextseq 500 mid Output run本研究针对前列腺癌PC3细胞来源的细胞外囊泡（extracellular vesicles）内的微小RNA（microRNA, miRNA）与信使RNA（messenger RNA, mRNA）进行了分析。 Kaighn ME等。《人前列腺癌细胞系（PC-3）的建立与鉴定》，《Invest. Urol.》，1979年，第17卷：16-23页。PubMed编号：447482 实验整体设计：采用Illumina Nextseq 500中通量输出运行模式，对PC3细胞外囊泡中的miRNA与mRNA进行双重复RNA测序（RNA sequencing, RNAseq）检测。