Cloning of two NLR genes at YRTD121 locus from wild emmer wheat Raw sequence reads. Triticum turgidum subsp. durum x Triticum dicoccoides

we use map-based cloning and long-read sequencing to isolate two nucleotide-binding and leucine-rich repeat (NLR) receptors from wild emmer wheat. By mutagenesis, gene silencing, and genetic transformation, we show that genes TdNLR1 and TdNLR2 oriented head-to-head are both required for YrTD121-mediated stripe rust resistance.

本研究采用图位克隆（map-based cloning）与长读长测序（long-read sequencing）技术，从野生二粒小麦中分离得到两种核苷酸结合富亮氨酸重复（NLR）受体。通过诱变、基因沉默与遗传转化实验，本研究证实，头对头定向排列的TdNLR1与TdNLR2基因，均为YrTD121介导的条锈病抗性所必需。