Essential genes whose expression is repressed by CTR9 depletion.

Genes were selected by the following criteria: (a) mRNA decreased more than 20-fold by CTR9 RNAi and (b) A significant decrease of at least 5-fold in representation in the RNAi library, using bloodstream forms either 3 or 6 days after tetracycline addition [48]. “rpkm” is the value for wild-type cells from RNASeq: an rpkm value of 20 is equivalent to one mRNA per cell [30]. “RNAi” is the rpkm for CTR9 RNAi divided by that for wild-type. “BS3” indicates the representation of this gene in RITSeq analysis in bloodstream forms, at day 3 after RNAi induction, divided by the representation without tetracycline; *not significant [48]. “BS6” is the same for 6 days of tetracycline.

研究人员依据以下标准筛选目标基因：(a) 经CTR9 RNA干扰（RNAi）处理后，其信使RNA（mRNA）表达水平下调超过20倍；(b) 在RNA干扰文库中的占比出现至少5倍的显著下降，实验采用添加四环素（tetracycline）后3天或6天的血流型（bloodstream forms）样本[48]。"rpkm"指野生型细胞经RNA测序（RNASeq）得到的数值：当rpkm值为20时，对应每个细胞中存在1条mRNA[30]。此处提及的"RNAi"为CTR9 RNAi处理组的rpkm值与野生型组rpkm值的比值。"BS3"表示该基因在血流型样本的RITSeq分析中的相对丰度，即RNA干扰诱导后第3天的基因代表丰度与未添加四环素时的丰度的比值，该结果无统计学显著性[48]。"BS6"的定义与BS3一致，对应四环素诱导6天的样本。