Table_1_Efficient Treatment of Sporothrix globosa Infection Using the Antibody Elicited by Recombinant Phage Nanofibers.DOCX

Antifungal therapy is used to treat sporotrichosis. However, there are several limitations in this therapy, such as development of drug resistance and potential health risks including liver injury. The purpose of our study was to evaluate the antifungal efficacy of antibody against the hybrid phage nanofibers displaying KPVQHALLTPLGLDR (phage-KR) in a fungal-infected mouse model. In this study, we extracte an antibody against hybrid phage nanofibers (phage-KR) from immunized mice and passively inoculate Sporothrix globosa (S. globosa) infected mice. The study shows that the antibody exhibits efficient inhibition efficacy of the S. globosa infection, including reduction of the progressive fungi colonizing, increase of animal survival rate and relief of organ inflammation in the mice. The results indicate that antibody against phage-KR may act as a potential strategy for safe and efficient treatment of S. globosa infections.

抗真菌疗法常用于治疗孢子丝菌病，但该疗法存在诸多局限，例如耐药性的产生以及包括肝损伤在内的潜在健康风险。本研究旨在评估针对展示KPVQHALLTPLGLDR序列的杂交噬菌体纳米纤维（phage-KR）的抗体，在真菌感染小鼠模型中的抗真菌功效。本研究从免疫小鼠体内分离得到针对杂交噬菌体纳米纤维（phage-KR）的抗体，并将其被动接种至感染了球形孢子丝菌（Sporothrix globosa，简称S. globosa）的小鼠体内。研究结果显示，该抗体可有效抑制球形孢子丝菌感染，具体表现为降低小鼠体内真菌定植负荷、提升动物存活率以及缓解器官炎症反应。上述结果表明，针对phage-KR的抗体有望成为一种安全高效治疗球形孢子丝菌感染的潜在策略。