STING facilitates vascular calcification via p-STAT1/NLRP3 signal. STING facilitates vascular calcification via p-STAT1/NLRP3 signal

Vascular calcification contributes to the cardiovascular morbidity and mortality of chronic kidney disease (CKD), but there is no approved treatment for vascular calcification. In this study, we report the role of STING in vascular calcification. To further investigate the molecular mechanism by which STING participates in vascular calcification, we performed high-throughput RNA-seq to identify the target gene of STING. Overall design: Primary mouse vascular smooth muscle cells from wild type and STING-/- mice were cultured in calcifying medium (CM). RNA-seq was performed to determine the downstream target of STING.

血管钙化可增加慢性肾脏病（CKD）患者的心血管疾病发病风险与死亡风险，目前尚无获批用于血管钙化的治疗方案。本研究探讨了干扰素基因刺激因子（STING）在血管钙化中的作用。为进一步探究STING参与血管钙化的分子机制，本研究通过高通量RNA测序（high-throughput RNA-seq）鉴定STING的靶基因。实验设计概述：提取野生型小鼠及STING敲除（STING-/-）小鼠的原代血管平滑肌细胞，将其置于钙化培养基（CM）中培养；随后通过RNA测序明确STING的下游靶基因。