Glioma LSM2 knockdown sequencing data

The human glioblastoma T98G cell line was cultured in DMEM medium supplemented with 10% fetal bovine serum and antibiotics. LSM2 gene knockdown was achieved using siRNA transfection with Lipofectamine RNAiMAX, and transfection efficiency was assessed by Western blotting and RT-qPCR. The experiment included three experimental groups (Group 1, 2, and 3) and three control group. After transfection, total RNA was extracted, rRNA was removed, and RNA-Seq libraries were prepared. High-throughput sequencing was performed on the Illumina NovaSeq 6000 system. The transcriptional effects of LSM2 knockdown were evaluated using GAPDH as a reference gene.

本研究中的人胶质母细胞瘤T98G细胞系（human glioblastoma T98G cell line）培养于添加10%胎牛血清与抗生素的DMEM培养基中。通过Lipofectamine RNAiMAX转染小干扰RNA（siRNA）实现LSM2基因敲低，并采用蛋白质印迹法（Western blotting）与实时定量聚合酶链反应（RT-qPCR）评估转染效率。本实验设置3个实验组（第1组、第2组、第3组）与3个对照组。转染完成后，提取总RNA并去除核糖体RNA（rRNA），随后构建RNA测序（RNA-Seq）文库，在Illumina NovaSeq 6000系统上完成高通量测序。以GAPDH作为内参基因，评估LSM2基因敲低的转录组学效应。