Single nuclear RNASeq Analysis of Anaplastic and Favourable Histology Hu_Wilms Tumor

Wilms tumor is the most common pediatric kidney cancer. The best predictor of clinical outcome for Wilms tumor patients is how their tumor looks under the microscope (histology). Usually, the resistant/anaplastic component of the tumor makes up only a fraction of the total number of cells in the cancer. A critical barrier to understanding therapeutic resistance in this disease is that studies performed to sequence the resistant component is diluted by the other components of the tumor.Therefore, the current proposal aims to using single-nuclear-RNA sequencing to isolate the gene expression patterns of individual cell types in Wilms tumor and to focus on the anaplastic/resistant cells. Overall design: Patient frozen tumor was analyzed using single cell nuclear RNASeq. The single cell nucleus was isolated and Single nuclear RNAseq was performed using 10X genomics -Single cell gene expression kit V3.

肾母细胞瘤（Wilms tumor）是儿童最常见的肾脏恶性肿瘤。肾母细胞瘤患者的临床结局最佳预测因子为肿瘤的显微镜下形态特征（组织学，histology）。通常，肿瘤的耐药/间变性成分仅占癌症总细胞数的极小一部分。解析该疾病治疗耐药性的关键障碍在于：针对耐药成分开展的测序研究常会被肿瘤的其他细胞成分所稀释。因此，本研究拟采用单核RNA测序（single-nuclear RNA sequencing）技术，解析肾母细胞瘤中各类细胞类型的基因表达模式，并重点关注间变性/耐药性细胞。
整体实验设计：对患者的冷冻肿瘤样本进行单细胞核RNA测序分析。首先分离单个细胞核，随后使用10X Genomics单细胞基因表达试剂盒V3完成单核RNA测序。