Transcriptional profiling of bulk tumor samples from implanted T cell low and T cell high mouse pancreatic tumors

Purpose: Use RNA-seq strategy to characterize the differentially expressed genes in T cell low and high mouse pancreatic tumors in order to identify factors contributing to the differential abundance of tumor infiltrating T cells. Methods: Subcutaneously implanted T cell low and high pancreatic tumors were utilized for the preparation of RNA-seq libraries from both bulk tumors. Examination of differentially expressed genes between two biological groups (T cell low v.s. T cell high tumors).

研究目的：采用RNA测序（RNA-seq）技术对T细胞低浸润与高浸润小鼠胰腺肿瘤中的差异表达基因（differentially expressed genes）进行表征，以鉴定导致肿瘤浸润T细胞差异富集的相关因子。研究方法：构建皮下移植的T细胞低浸润与高浸润胰腺肿瘤模型，从两类整块肿瘤组织中制备RNA-seq文库，并对两个生物学组别（T细胞低浸润组与T细胞高浸润组肿瘤）之间的差异表达基因进行分析。