RNA-seq profiles of pluripotent and surface ectoderm cells with and without p63. RNA-seq profiles of pluripotent and surface ectoderm cells with and without p63

Purpose: Evaluate p63 regulation of gene expression in human embryonic stem cells and the surface ectoderm Methods: RNA profiles for human embyronic stem cells and surface ectoderm cells with and without p63 using deep sequencing, in duplicate, on Illumina NextSeq 500 sequencer. Quality of reads were checked by fastqc. Reads were aligned to hg19 genome using tophat and FPKM values were generated using Homer. Results: p63 is largely unable to regulate gene expression in pluripotent cells, but acts primarily as a repressor in the surface ectoderm. Conclusion: p63 regulation of gene expression is dependent on the changes the cell undergoes during surface ectoderm commitment Overall design: RNA-seq profiles of human embryonic stem cells and surface ectoderm cells with and without p63 were generated in duplicate by deep sequencing on Illumina NextSeq 500 sequencer. ***Please note that the submitters no longer have access to the fastq or all of the original bam files (i.e. no raw data). The text files used for all downstream analyses were provided.

数据集用途：评估p63对人类胚胎干细胞及表面外胚层细胞基因表达的调控功能。实验方法：采用Illumina NextSeq 500测序仪，对存在p63与缺失p63的人类胚胎干细胞、表面外胚层细胞开展双重复深度测序，获取RNA表达谱；通过FastQC检测测序reads的质量；使用Tophat将reads比对至hg19参考基因组，并通过Homer软件计算生成FPKM值。实验结果：p63在多能干细胞中基本无法实现基因表达调控，而主要在表面外胚层细胞中作为转录抑制因子发挥作用。研究结论：p63对基因表达的调控作用依赖于细胞向表面外胚层定向分化过程中发生的状态改变。整体实验设计：通过Illumina NextSeq 500测序仪进行双重复深度测序，获取存在p63与缺失p63的人类胚胎干细胞、表面外胚层细胞的RNA-seq表达谱。***请注意：提交者已无法获取fastq文件及全部原始bam文件（即无原始测序数据），仅提供了用于所有下游分析的文本文件。