2_Experimental protocols for PRIN project RiWeCIS

This repository contains experimental protocols developed and used within the PRIN project “RiWeCIS”, funded by the Italian Ministry of University and Research (MUR). Due to Zenodo platform limitations, the repository is provided as a compressed (.zip) archive in order to preserve the original folder structure. Users are required to extract (unzip) the archive to properly access and navigate its contents. The repository includes protocols associated with different Work Packages (WP2 and WP3), detailing experimental procedures applied during the project. ----------------------------------------WP2 Protocols---------------------------------------- The repository contains the following protocols: - Plant growth protocol Description of the procedures for seed sowing and growth conditions of the plant species involved in the project. - VIGS protocol using TRV A detailed protocol describing Virus-Induced Gene Silencing (VIGS) using Tobacco Rattle Virus (TRV), including all steps from cloning to plant treatment, applied to *Solanum nigrum* and *Nicotiana benthamiana*. - VIGS protocol in *Echinochloa crus-galli* Protocol describing attempts to establish VIGS in *Echinochloa crus-galli* using: - Cucumber mosaic virus (CMV) - Barley stripe mosaic virus (BSMV) ----------------------------------------WP3 Protocol---------------------------------------- - Greenhouse treatment with nano-encapsulated herbicide This protocol describes greenhouse experiments conducted on *Oryza sativa* and *Echinochloa crus-galli*, aimed at evaluating the efficacy of nano-encapsulated imazamox. The protocol includes details on plant growth conditions, preparation and application of the nanoparticle-based formulation, treatment setup, and assessment of herbicide effects at the whole-plant level. ---------------------------------------- All protocols are provided to ensure reproducibility and transparency of the experimental procedures developed within the PRIN project “RiWeCIS”.本仓库包含由意大利大学与研究部（Ministero dell'Università e della Ricerca, MUR）资助的PRIN项目"RiWeCIS"内部开发并使用的实验规程。 鉴于Zenodo平台的限制，本仓库以压缩(.zip)归档形式提供，以保留原始文件夹结构。用户需解压该归档，方可正常访问并浏览其内容。 本仓库涵盖与不同工作包（Work Package, WP）2和WP3相关的规程，详述了项目实施期间采用的实验操作流程。 ----------------------------------------WP2 规程---------------------------------------- 本仓库包含的规程如下： - 植物生长规程：详述项目涉及植物物种的播种及生长条件操作流程。 - 基于烟草脆裂病毒（Tobacco Rattle Virus, TRV）的病毒诱导基因沉默（Virus-Induced Gene Silencing, VIGS）规程：详细描述采用烟草脆裂病毒（TRV）开展病毒诱导基因沉默（VIGS）的完整操作流程，涵盖从克隆到植物处理的全部步骤，适用于龙葵（*Solanum nigrum*）和本氏烟（*Nicotiana benthamiana*）。 - 稗草（*Echinochloa crus-galli*）病毒诱导基因沉默（VIGS）尝试规程：详述采用以下病毒在稗草（*Echinochloa crus-galli*）中建立VIGS体系的尝试： - 黄瓜花叶病毒（Cucumber mosaic virus, CMV） - 大麦条纹花叶病毒（Barley stripe mosaic virus, BSMV） ----------------------------------------WP3 规程---------------------------------------- - 纳米包埋除草剂温室处理规程：本规程详述了在水稻（*Oryza sativa*）和稗草（*Echinochloa crus-galli*）上开展的温室实验，旨在评估纳米包埋咪唑喹啉酸（imazamox）的除草效果。本规程包含植物生长条件、纳米颗粒制剂的制备与施用、处理设置以及整株水平除草剂效应评估的详细信息。 所有规程均已提供，以确保PRIN项目"RiWeCIS"内部开发的实验操作流程具备可重复性与透明度。