Here, we present a large RNA-Seq dataset of Human alphaherpesvirus 1, derived from long-read sequencing. Pacific Biosciences Sequel platform and a Oxford Nanopore's MinION device with direct RNA sequencing and cDNA sequencing methods were used.

Here, we present a large RNA-Seq dataset of Human alphaherpesvirus 1, derived from long-read sequencing. Pacific Biosciences Sequel platform and a Oxford Nanopore's MinION device with direct RNA sequencing and cDNA sequencing methods were used.PolyA or CAP selected or ribodepleted mRNAs were were isolated from different time point after viral infection and reverse transcriped into cDNA and using specific or random primers.

本研究发布一套基于长读长测序技术构建的人类α疱疹病毒1型（Human alphaherpesvirus 1）大规模RNA测序（RNA-Seq）数据集。该数据集采用太平洋生物科学Sequel测序平台（Pacific Biosciences Sequel）与牛津纳米孔MinION测序设备（Oxford Nanopore's MinION），运用直接RNA测序与cDNA测序两种实验策略。研究人员在病毒感染后的不同时间点分离经PolyA富集、CAP帽选择或核糖去除处理的mRNA，通过特异性引物或随机引物将其反转录为cDNA。