Fetus-derived IGF2 matches placental development to fetal demand II [RNA-seq]

We used transcriptome analysis to identify genes transcriptionally disregulated in feto-placental endothelial cells from mutants with conditional deletion of Igf2 gene driven by the Tek-cre. Overall design: RNA-seq in FACS-sorted feto-placental endothelial cells isolated from E16 Igf2ECKO mutants (n=4) and littermate controls (n=4)

本研究采用转录组分析（transcriptome analysis）技术，筛选以Tek-Cre为驱动工具的Igf2基因（胰岛素样生长因子2, Igf2）条件性敲除突变体的胎儿胎盘内皮细胞内的转录失调基因。实验设计概述：对胚胎期16天（E16）的Igf2ECKO突变体（n=4）及其同窝对照小鼠（n=4）中分离得到的、经荧光激活细胞分选（Fluorescence Activated Cell Sorting, FACS）纯化的胎儿胎盘内皮细胞开展RNA测序（RNA-seq）。