ArcA Chip-chip from Escherichia coli MG1655 K-12, WT and ∆arcA strains

Mapping the occupancy of ArcA throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anaerobic and aerobic growth conditions. As a control, we also performed ChIP-chip onArcA in a ∆arcA mutant strain of Escherchia coli MG1655 K-12. Described in the manuscript The response regulator ArcA uses a diverse binding site architechture to globally regulate carbon oxidation in E. coli Mapping of occupancy of ArcA in the genome of Escherchia coli MG1655 K-12 during anaerobic fermentation and aerobic respiration. Immunoprecipitated DNA compared to INPUT for each sample.

本研究采用亲和纯化抗体，在厌氧及有氧生长条件下，绘制了大肠杆菌MG1655 K-12全基因组范围内ArcA的结合占据图谱。作为对照，本研究同时对大肠杆菌MG1655 K-12的ΔarcA缺失突变菌株开展了ArcA的染色质免疫沉淀芯片（ChIP-chip）实验。本数据集相关内容已在论文《响应调节因子ArcA通过多样化结合位点结构全局调控大肠杆菌碳氧化过程》中进行了详细说明，该研究同时绘制了大肠杆菌MG1655 K-12在厌氧发酵与有氧呼吸阶段全基因组范围内ArcA的结合占据图谱。针对各样本的免疫沉淀DNA与INPUT对照样本进行了比对分析。