Bronchial epithelial cells accessibility upon flagellin stimulation (ATAC-Seq)

Innate immune memory is a new concept describing ability of innate myeloid and lymphoid cells to gain memory characteristics after transient stimulation, resulting in an nonspecific modified response upon secondary challenge. In recent work, we demonstrated that bronchial epithelial cells, non immune cells, that are the first immune cells of the lower respiratory tract to encounter pathogens were capable of innate immune memory after pre-exposure to Pseudomonas aeruginosa flagellin. In the present study we identified chromatin modifications induced by flagellin pre-exposure that sustain reprogramming of transcriptional patterns. We researched chromatin structure modifications responsible for the modulation of gene expression and thus of innate memory induced by the exposure of cells to flagellin. Overall design: 15 samples, 5 conditions in triplicate

固有免疫记忆（innate immune memory）是一类新兴概念，用以描述固有髓系与淋巴系细胞在短暂刺激后获得记忆特性，并在二次刺激挑战时产生非特异性修饰应答的能力。既往研究中，我们证实：作为下呼吸道最先接触病原体的免疫样细胞，支气管上皮细胞（一种非免疫细胞）在预先暴露于铜绿假单胞菌（Pseudomonas aeruginosa）鞭毛蛋白后，可产生固有免疫记忆。本研究中，我们鉴定出由鞭毛蛋白预暴露诱导的、可维持转录模式重编程的染色质修饰。我们针对细胞暴露于鞭毛蛋白后诱导固有免疫记忆所依赖的基因表达调控相关染色质结构修饰展开了研究。实验整体设计：共15个样本，包含5种实验条件，每种条件设置3次生物学重复。