Histone deacetylation primes chromatin to preserve epigenetic memory for self-propagation of heterochromatin domains [Clr3-CDx2]. Histone deacetylation primes chromatin to preserve epigenetic memory for self-propagation of heterochromatin domains [Clr3-CDx2]

Clr3-CDx2 occupancy along fission yeast chromosomes. Chromodomain-mediated loading targets Clr3-CDx2 to heterochromatin domains. Overall design: DNA co-purified with Clr3-CDx2 chromatin was random-prime PCR amplified and analyzed using custom 60mer Agilent array that tiles Schizosaccharomyces pombe genome in 300bp intervals.

裂殖酵母染色体上Clr3-CDx2的结合占据谱。染色质结构域（Chromodomain）介导的加载过程可将Clr3-CDx2靶向招募至异染色质区域。整体实验设计：与Clr3-CDx2染色质共纯化所得的DNA经随机引物PCR扩增后，采用定制60聚体安捷伦（Agilent）芯片进行检测分析，该芯片以300bp的间隔铺列覆盖粟酒裂殖酵母（Schizosaccharomyces pombe）的全基因组。