Epigenomic and transcriptomic features induced by silencing of ZNF280C in colon cancer cells and knock-out of Zfp280c in mice [ChIP-Seq, ATAC-Seq]. Epigenomic and transcriptomic features induced by silencing of ZNF280C in colon cancer cells and knock-out of Zfp280c in mice [ChIP-Seq, ATAC-Seq]

Znic finger proteins play crucial roles in development and disease, including tumorigenesis. Here, we identifed ZNF280C as a novel oncogenic driver in colorectal cancer, and systematically studied the molecular mechanisms underlying ZNF280C-mediated malignant transformation and progression. Overall design: RKO colon cancer cells stably expressing a ZNF280C-targeting shRNA controlled by a tetracycline-inducible promoter were treated with or without 1 ug/ml doxycycline for 72 hours, and the cells were harvested for analysis as described below. For transgenic mice experiments, Zfp280c knock-out or wild-type C57BL/6J mice were sacrificed at 12 weeks of age and colon tissues were havested for transriptomic analysis as described below.

锌指蛋白（Zinc finger proteins）在发育及包括肿瘤发生在内的多种疾病进程中发挥关键调控作用。本研究将ZNF280C鉴定为结直肠癌中的新型致癌驱动因子，并系统解析了ZNF280C介导的恶性转化与疾病进展的分子机制。
总体实验设计：将稳定表达受四环素诱导型启动子调控的ZNF280C靶向短发夹RNA（short hairpin RNA，shRNA）的RKO结肠癌细胞，分别以1 μg/ml多西环素（doxycycline）处理与不予处理，培养72小时后收集细胞，按照下文所述方法进行分析。
转基因小鼠实验：于12周龄时处死Zfp280c敲除型或野生型C57BL/6J小鼠，采集结肠组织，按照下文所述方法进行转录组（transcriptomic）分析。