Replication data for: Complete genome sequence of a potyvirus infecting yam beans (Pachyrhizus spp.) in Peru

Towards the end of 2010, viral disease symptoms (mosaic with or without leaf deformation) were observed in a yam bean field trial at the CIP research station in San Ramon, Junin, Peru (wet tropics, 800 masl). The disease could be graft transmitted, and based on symptoms, an infection by the potyvirus bean common mosaic virus (BCMV) was suspected. Plants were tested by NCM-ELISA using potyvirus-specific antibodies (Agdia CAB 27200), to which all symptomatic plants reacted positively, whereas symptomless greenhouse-grown plants did not react. Small interfering RNA (siRNA) sequencing (Provider: Fasteris Life Sciences SA, Plan-les-Ouates, Switzerland) and assembly were performed on a sample (P. tuberosus accession CIP209014) showing severe mosaic and leaf deformation as described by Kreuze et al., except that the reads were assembled using Velvet’s Assembly Assembler (v1.1)

2010年末，在秘鲁胡宁省圣拉蒙的CIP研究站（湿热带地区，海拔800米）的豆薯田间试验中，观察到植株出现病毒性病害症状（花叶，伴或不伴叶片变形）。该病害可通过嫁接传播，基于症状推测为马铃薯Y病毒属的菜豆普通花叶病毒（BCMV）感染。采用马铃薯Y病毒属特异性抗体（Agdia CAB 27200）的NCM-ELISA检测显示，所有表现症状的植株均呈阳性反应，而温室中生长的无症状植株则无反应。对表现严重花叶和叶片变形的样本（P. tuberosus材料CIP209014）进行了小干扰RNA（siRNA）测序（提供者：瑞士Plan-les-Ouates的Fasteris Life Sciences SA公司）及组装，方法参考Kreuze等人，唯reads组装采用Velvet的Assembly Assembler（v1.1）软件。