NEON Biorepository Zooplankton Collection (Unsorted Bulk Sample)

This collection contains unsorted bulk zooplankton samples (NEON sample class: zoo_fieldData_in.sampleID.dna). Bulk zooplankton samples are collected using field sterile methods at lake sites during biology bout windows in spring and fall. Samples are collected using either a tow net (water deeper than 4 m) or a Schindler-Patalas trap (water shallower than 4 m) depending on the depth at the sampling location. Samples are collected near the NEON profiling buoy as well as the littoral sensor sets. During each sampling bout, three samples are collected for morphological taxonomy and three samples for DNA/bulk archiving. The DNA metabarcoding samples from bout 2 (summer) are sent to an external laboratory for metabarcoding. The unsorted bulk samples collected using field sterile methods from bout 1 (spring) and bout 3 (autumn) are archived for future research. Unsorted bulk zooplankton samples are preserved in 30-40% ethanol (prior to 2020) or 95% ethanol (2020 and beyond) and archived in a temperature (17-18°C) and humidity controlled environment. See related links below for protocols and NEON related data products.

该数据集包含未分类的浮游动物批量样本（NEON样本类别：zoo_fieldData_in.sampleID.dna）。浮游动物批量样本采用野外无菌方法采集，采集地点为湖泊站点，时间为春季和秋季的生物学采样窗口期。根据采样点水深，样本采集工具选择拖网（水深>4米）或Schindler-Patalas陷阱（水深<4米）。样本采集于NEON剖面浮标附近及沿岸传感器组区域。每次采样期间，采集3份样本用于形态分类学研究，3份样本用于DNA/批量存档。第2期（夏季）的DNA宏条形码（DNA metabarcoding）样本被送往外部实验室进行宏条形码分析。采用野外无菌方法采集的第1期（春季）和第3期（秋季）未分类批量样本被存档以供未来研究。未分类的浮游动物批量样本采用30-40%乙醇（2020年前）或95%乙醇（2020年及以后）保存，并存放于温湿度可控环境中（温度17-18°C）。有关实验方案及NEON相关数据产品，请参见下方相关链接。