Isolation and characterization of gene sequences expressed in cotton fiber

ABSTRACT Cotton fiber are tubular cells which develop from the differentiation of ovule epidermis. In addition to being one of the most important natural fiber of the textile group, cotton fiber afford an excellent experimental system for studying the cell wall. The aim of this work was to isolate and characterise the genes expressed in cotton fiber (Gossypium hirsutum L.) to be used in future work in cotton breeding. Fiber of the cotton cultivar CNPA ITA 90 II were used to extract RNA for the subsequent generation of a cDNA library. Seventeen sequences were obtained, of which 14 were already described in the NCBI database (National Centre for Biotechnology Information), such as those encoding the lipid transfer proteins (LTPs) and arabinogalactans (AGP). However, other cDNAs such as the B05 clone, which displays homology with the glycosyltransferases, have still not been described for this crop. Nevertheless, results showed that several clones obtained in this study are associated with cell wall proteins, wall-modifying enzymes and lipid transfer proteins directly involved in fiber development.

摘要：棉纤维是由胚珠表皮细胞分化发育而来的管状细胞。其不仅是纺织产业最重要的天然纤维之一，同时也是研究植物细胞壁的优质实验模型体系。本研究旨在分离并鉴定陆地棉（Gossypium hirsutum L.）纤维中表达的基因，为后续棉花育种研究提供材料。本研究选用棉花品种CNPA ITA 90 II的纤维组织提取RNA，进而构建cDNA文库。共获得17条序列，其中14条已在NCBI（National Centre for Biotechnology Information，美国国家生物技术信息中心）数据库中被注释，包括编码脂质转运蛋白（lipid transfer proteins, LTPs）与阿拉伯半乳聚糖蛋白（arabinogalactans, AGPs）的序列。但另有部分cDNA克隆（如B05克隆）与糖基转移酶具有同源性，这类序列在该作物中尚未见报道。不过，本研究获得的多个克隆均与细胞壁蛋白、细胞壁修饰酶以及直接参与纤维发育的脂质转运蛋白密切相关。