Identification of a Broadly Fibrogenic Macrophage Subset Induced by Type 3 Inflammation. Identification of a Broadly Fibrogenic Macrophage Subset Induced by Type 3 Inflammation

Cross-sectional single cell RNA sequencing (scRNAseq) of hepatic cells enzymatically isolated from murine models of toxin- (CCl4) or diet-induced (Gubra Amlyn Nash or GAN) liver fibrosis to identify macrophage populations associated with fibrosis. Overall design: Cells were enzymatically isolated from murine livers at the peak of fibrosis (see paper for details), FACS sorted to remove dead cells and fixed in methanol before analysis using 10x Genomics scRNAseq platform. For samples labeled MMPNeg or MMPPos, cells were additionally FACS sorted based on signal from the MMPsense dye which distinguishes active intracellular matrix metalloproteases (MMPs).

本数据集为针对肝纤维化小鼠模型中酶解法分离得到的肝细胞所开展的横断面单细胞RNA测序（single cell RNA sequencing, scRNAseq），所用小鼠模型包括毒素诱导（四氯化碳，CCl4）型与膳食诱导型（Gubra Amlyn Nash，简称GAN）肝纤维化模型，旨在鉴定与纤维化相关的巨噬细胞群。整体实验设计：在纤维化进程峰值阶段，通过酶解法分离小鼠肝脏细胞（详见论文原文），经荧光激活细胞分选术（fluorescence-activated cell sorting, FACS）去除死细胞后以甲醇固定，随后使用10x Genomics单细胞RNA测序平台开展检测。对于标记为MMPNeg或MMPPos的样本，额外基于MMPsense染料的信号进行FACS分选，该染料可区分具有活性的胞内基质金属蛋白酶（matrix metalloproteases, MMPs）。