RNA-seq Analysis of intestinal tubes from the Unfed and Fed Xenopus laevis tadpoles

In this study, we assessed nutrient-dependent organ morphogenesis in Xenopus laevis. We performed the RNA-seq analysis of the gastrointestinal (GI) tract for both fed and unfed tadpoles to identify feeding-dependent inter-organ transmitters secreted from GI tract post-prandially during development. We found that one of the GI hormones, glucose-dependent insulinotropic polypeptide (GIP), is required for thyroid morphogenesis. Overall design: Intestinal mRNA profiles of the unfed and fed Xenopus laevis tadpoles (10 dpf to 16 dpf, raised at 19 ?) contributor: Bioengineering Lab (https://gikenbio.com/)

本研究针对非洲爪蟾（Xenopus laevis）开展营养依赖型器官形态发生过程的相关评估。我们对摄食组与禁食组的非洲爪蟾蝌蚪的胃肠道（gastrointestinal tract，简称GI tract）开展RNA测序（RNA-seq）分析，以鉴定发育过程中摄食后由胃肠道分泌的、依赖于摄食的器官间传递因子。研究发现，胃肠道激素之一的葡萄糖依赖性促胰岛素多肽（glucose-dependent insulinotropic polypeptide，GIP）是甲状腺形态发生所必需的调控因子。实验设计：对受精后天数（days post fertilization，dpf）10至16天、于19℃环境下饲养的禁食与摄食组非洲爪蟾蝌蚪的肠道mRNA表达谱进行检测。贡献单位：生物工程实验室（Bioengineering Lab，https://gikenbio.com/）