Revisiting the gastrin-releasing peptide/bombesin system: A reverse-evolutionary study considering Xenopus

The expression levels of gastrin-releasing peptide (GRP) and GRP receptor (GRPR) mRNA in Xenopus central nervous system were measured by the real-time quantitative PCR. Relative expression levels of GRP and GRPR in the central nervous system: the telencephalon, the diencephalon/mesencephalon/pons/cerebellum, the medulla oblongata, and the spinal cord of males and females were analyzed. As internal control gene, a gene for Drosha was used. The Table (Fig5_data.xlsx) is raw data which show Cq value of real time PCR and the source of Figure 5 in the manuscript "Revisiting the gastrin-releasing peptide/bombesin system: A reverse-evolutionary study considering Xenopus".

本研究采用实时定量PCR（real-time quantitative PCR）检测了爪蟾（Xenopus）中枢神经系统内胃泌素释放肽（gastrin-releasing peptide，GRP）及其受体GRPR（GRP receptor，GRPR）的mRNA表达水平。本研究进一步分析了雌雄个体中枢神经系统各脑区（端脑、间脑、中脑、脑桥与小脑、延髓及脊髓）中GRP与GRPR的相对表达水平。本研究以Drosha基因作为内参基因。附件文件Fig5_data.xlsx为原始数据文件，包含实时定量PCR的Cq值，同时为本论文《重新审视胃泌素释放肽/铃蟾肽系统：一项以爪蟾为对象的逆向进化研究》（原文标题：Revisiting the gastrin-releasing peptide/bombesin system: A reverse-evolutionary study considering Xenopus）中的图5提供了原始数据支撑。