NanoString miRNA profiling of peripheral blood mononuclear cells from HIV-1-infected elite suppressors, viremic patients, and uninfected control donors. Homo sapiens

This study used the NanoString nCounter hybridization system and nCounter miRNA expression assays to identify and quantitate circulating cellular miRNAs during HIV-1 elite suppression, active HIV-1 replication, and uninfected status. Overall design: Blood samples were from eight uninfected controls, seven HIV-1 elite suppressors with undetectable viral load, and six viremic HIV-1-infected patients.

本研究采用NanoString nCounter杂交系统（NanoString nCounter hybridization system）与nCounter miRNA表达分析试剂盒（nCounter miRNA expression assays），对HIV-1精英抑制状态、活动性HIV-1复制状态以及未感染健康状态下的循环细胞microRNA（miRNA）进行鉴定与定量分析。实验整体设计：血液样本取自8名未感染健康对照者、7名病毒载量不可检测的HIV-1精英抑制者，以及6名伴病毒血症的HIV-1感染患者。