Galectin-8 downmodulates TLR4 activation and impairs bacterial clearance in a mouse model of Pseudomonas keratitis

In our study, we used a mouse model infected with Pseudomonas aeruginosa (PA) to provoke a painful, sight-threatening corneal infection. FACS sorted WT and Gal-8-/- neutrophils of PA infected corneas were processed for next-generation transcriptomic RNA sequencing (RNA-seq).

本研究中，我们使用感染铜绿假单胞菌（Pseudomonas aeruginosa, PA）的小鼠模型，诱发了疼痛性且威胁视力的角膜感染。对感染PA的角膜中经荧光激活细胞分选（FACS）得到的野生型（WT）与半乳糖凝集素-8基因敲除（Gal-8-/-）中性粒细胞，进行下一代转录组RNA测序（RNA-seq）。