Comparison between lncRNA-HAL silenced MCF7 cells and control shLuc MCF7 cells

Analysis of differentially expressed genes in MCF7 cancer cells stably silenced with shlncHAL-2 short-hairpin versus control MCF7 cells stably expressing control shLuc short-hairpin. Overall design: Two groups were analyzed in triplicate or quadruplicate. The first group was MCF7 stably transfected with a vector expressing shlnc-HAL-2 for lncRNA-HAL knock-down and the second group was an MCF7 cell line stably transfected with a vector expressing shLuc control hairpin.

本数据集针对经shlncHAL-2短发夹RNA（short-hairpin RNA）稳定沉默的MCF7癌细胞，与稳定表达对照shLuc短发夹RNA的MCF7对照细胞之间的差异表达基因展开分析。整体实验设计：设置两组样本，分别进行三次生物学重复或四次生物学重复。第一组为经携带shlnc-HAL-2载体稳定转染的MCF7细胞，用于敲低lncRNA-HAL；第二组为经携带shLuc对照发夹结构载体稳定转染的MCF7细胞系。