Combined Proteomics and Transcriptomics Identifies Serpin Family C Member 1 Associated Protein as a Biomarker of Endometriosis

Objective: To explore potential biomarkers indicating endometriosis. Results: A sum of 979 mRNAs and 39 proteins were tested to be significantly differentially expression in the standard cluster compared with the endometriosis cluster. Venn analysis showed a filtered signature of only two down-regulated molecules in the EM group, i.e., fetuin B and serpin family C member 1; the latter showed a big variance between the control category and the EM set in the authentication test. Conclusion: Serpin family C member 1 may be a useful possible biomarker for the analysis of EM. Overall design: Materials and methods: A proteomics method and combined quantitative transcriptomics was adopted to highlight markers in the Endometriosis (EM). Venn analysis was used to integrate the ribonucleic acid sequencing (RNA-seq) and protein profiles. Promising candidate markers were tested by enzyme-related immunosorbent assay.

研究目标：探索可提示子宫内膜异位症的潜在生物标志物。研究结果：经检测，标准聚类组与子宫内膜异位症聚类组间共有979个信使RNA（mRNA）及39个蛋白质呈显著差异表达。Venn分析显示，经筛选得到的特征分子仅包含2个在EM组中下调的分子，即胎球蛋白B（fetuin B）与丝氨酸蛋白酶抑制剂家族C成员1（serpin family C member 1）；在验证实验中，后者在对照组与EM组间呈现出显著的表达差异。研究结论：丝氨酸蛋白酶抑制剂家族C成员1或可成为用于子宫内膜异位症分析的潜在有效生物标志物。整体实验设计：材料与方法：本研究采用蛋白质组学方法结合定量转录组学技术，筛选子宫内膜异位症（Endometriosis，简称EM）相关标志物；通过Venn分析整合核糖核酸测序（RNA-seq）与蛋白质表达谱数据；采用酶联免疫吸附实验对候选标志物进行验证检测。