Control of Embryonic Stem Cell State by Mediator and Cohesin (Illumina ChIP-Seq data). Mus musculus

The key transcription factors that control the embryonic stem cell gene expression program have been identified, but how they function to implement this program is not well understood. While screening for genes essential for maintenance of ES cell state, we identified many components of the Mediator and Cohesin complexes. Mediator and Cohesin were found to physically and functionally connect the enhancers and core promoters of active genes. An ES cell Mediator complex was found to copurify with Cohesin and its loading factor Nipbl, and normal levels of these proteins were essential for expression of the genes they occupy and for maintenance of ES cell state. Overall design: See associated publication. Sample GSM555166 (GSE22320) was used as a negative control.

目前已明确调控胚胎干细胞基因表达程序的关键转录因子，但对于这些因子如何执行该基因表达程序的具体机制，学界仍尚未完全阐明。在针对维持胚胎干细胞（ES cell）状态所必需的基因进行筛选时，我们鉴定出了中介体（Mediator）与黏连蛋白（Cohesin）复合物的多种组分。研究发现，中介体与黏连蛋白可在物理层面与功能层面同时连接活跃基因的增强子与核心启动子。进一步研究显示，胚胎干细胞中的中介体复合物可与黏连蛋白及其加载因子Nipbl发生共纯化现象，且上述蛋白的正常水平，对于其所结合基因的表达以及胚胎干细胞状态的维持均不可或缺。整体实验设计：详见相关关联出版物。样本GSM555166（GSE22320）被用作阴性对照。