The impact of CXCL5 overexpression on the primary tumor microenvironment of B16F1 melanomas

CXCL5, a strong neutrophil-chemoattractant, has been reportet to be expressed in different cancer entities with diverse outcomes in disease progression. Contradictory outcome in disease progression in different tumor entities might be explained by a tumor type specific expression pattern of chemokines, chemokine receptors and growth factors that act in concert with CXCL5. This study evaluates the impact of CXCL5 expression on the tumor mircoenvironment in a syngeneic mouse melanoma model. Overall design: 105 B16F1 and B16F1-CXCL5 murine melanoma were injected intradermally into the flank skin of C57BL/6 J mice. Primary tumors were grown up to 250-350mm³, excised, snap frozen and then processed for RNA sequencing.CXCL5作为一种强效中性粒细胞趋化因子（neutrophil-chemoattractant），已有研究证实其可在多种癌症实体中表达，并在疾病进展过程中呈现迥异的预后结局。不同肿瘤实体中出现的预后矛盾现象，可通过与CXCL5协同发挥作用的趋化因子、趋化因子受体及生长因子的肿瘤特异性表达模式加以解释。本研究评估了CXCL5表达对同源小鼠黑色素瘤模型肿瘤微环境的影响。 实验整体设计：将105株B16F1与B16F1-CXCL5小鼠黑色素瘤细胞皮内注射至C57BL/6 J小鼠的胁腹皮肤。待原发肿瘤生长至250~350mm³时，将其切除并快速冷冻，随后进行RNA测序。