Metatranscriptome high-resolution time series from InVirT-19-BATS

This resource contains data originating from the JGI project, "Inferring virus-host interactions through multi-omics high-resolution time series" (see award and files). The sequencing project was part of the NSF project, "InVirT-19-BATS": "Inferring Cellular Lysis and Regeneration of Organic Matter by Marine Viruses," which involved sample collection in the vicinity of the Bermuda Atlantic Time Series (BATS) in October 2019 (see the BCO-DMO project page). img_data_integration.md explains files in this record and shows how they were generated, including code which can be run as a reproducible workflow. img_data.tsv.gz contains gene-level data from assembled metatranscriptomic transcripts. The table merges data products generated by the JGI IMG Annotation Pipeline (v5.0.23). There are 103 metatranscriptomic samples that are included with sample metadata and CTD data. INVIRT19_metaT_metadata.txt contains metatranscriptomic sample metadata provided by Daniel Muratore that also applies to the samples in the Muratore et al. 2024 paper, "Diel partitioning in microbial phosphorus acquisition in the Sargasso Sea." all_casts.csv, also provided by Daniel Muratore, contains cast CTD data collected concomitantly with the metatranscriptomic bottle samples. cognames2003-2014.tab, tigr_names.txt, and ko_list.txt contain function names used in img_data.tsv. v6 is a slight revision of v5 with an updated version of dataset_meta_data.xlsx. v5 made changes relevant to submission of the dataset to Simons C-MAP, an oceanographic data repository with visualization capabilities. img_data_integration.md was renamed to ccomp_data_collation_from_img.md to reflect that we at C-CoMP developed a pipeline to collate sequencing datasets from JGI IMG in a single tabular file. dataset_meta_data.xlsx and vars_meta_data.xlsx are C-MAP metadata sheets required for the dataset.本数据集源自JGI（Joint Genome Institute，联合基因组研究所）项目“通过多组学高分辨率时间序列推断病毒-宿主相互作用”（详见奖项说明及相关文件）。该测序项目隶属于NSF（美国国家科学基金会，National Science Foundation）项目“InVirT-19-BATS”：“海洋病毒介导的细胞裂解与有机质再生推断”，项目组于2019年10月在百慕大大西洋时间序列（Bermuda Atlantic Time Series, BATS）海域附近完成样本采集（详见BCO-DMO（海洋生物与化学海洋学数据管理办公室，Biological and Chemical Oceanography Data Management Office）项目页面）。 `img_data_integration.md`对本数据集记录中的文件进行了解释，并说明了其生成流程，包含可复现工作流的代码。 `img_data.tsv.gz`包含来自组装后宏转录本的基因级数据。该表格整合了JGI IMG注释流程（IMG Annotation Pipeline，v5.0.23）生成的多组学数据产品。本数据集共包含103份宏转录组样本，附带样本元数据与CTD（电导率-温度-深度仪，Conductivity-Temperature-Depth）数据。 `INVIRT19_metaT_metadata.txt`包含由Daniel Muratore提供的宏转录组样本元数据，该元数据同样适用于Muratore等人2024年发表的论文《马尾藻海微生物磷获取的昼夜分区》中的样本。 `all_casts.csv`同样由Daniel Muratore提供，包含与宏转录组瓶采样本同步采集的CTD剖面数据。 `cognames2003-2014.tab`、`tigr_names.txt`与`ko_list.txt`包含`img_data.tsv`中使用的功能名称注释。 v6是v5的小幅修订版本，附带更新后的`dataset_meta_data.xlsx`。v5版本针对数据集提交至Simons C-MAP（具备可视化功能的海洋学数据仓储）做出了相关调整。原`img_data_integration.md`已更名为`ccomp_data_collation_from_img.md`，以体现C-CoMP团队开发了一套可将JGI IMG的测序数据集整合为单一表格文件的流程。`dataset_meta_data.xlsx`与`vars_meta_data.xlsx`为数据集提交至C-MAP所需的元数据表。