Global DNA methylation pattern of fibroblasts in idiopathic pulmonary fibrosis: relationship to gene expression

Analysis of Idiopathic pulmonary fibrosis (IPF) at gene expression level. The hypothesis tested in the present study was that Epigenetic mechanisms are likely to be associated with pathogenesis in IPF. To determine the DNA methylation change, and their effects on gene expression, we compared microarray data of DNA methylation and RNA expression. Results provide that among the genes whose DNA methylation status and RNA expression were both significantly altered between IPF-rapid and normal controls. Genomic DNA obtained from Idiopathic pulmonary fibrosis samples.

本研究针对特发性肺纤维化（Idiopathic Pulmonary Fibrosis, IPF）开展基因表达层面的分析。本研究验证的核心假说为：表观遗传机制极有可能与IPF的发病机制存在关联。为明确DNA甲基化的变化情况及其对基因表达的调控作用，本研究对比了DNA甲基化与RNA表达的微阵列数据。研究结果表明，在IPF快速进展组与正常对照组中，存在一批DNA甲基化状态与RNA表达水平均发生显著改变的基因。本研究所用基因组DNA提取自特发性肺纤维化样本。