Kinetics analysis of gene expression in CD4+ Th17 cells activated with cognate antigen. Kinetics analysis of gene expression in CD4+ Th17 cells activated with cognate antigen

Analysis of genes expressed at various time points after peptide specific TCR activation in vitro. The hypothesis tested was that activation genes would be upregulated at early times points (D2, D4) while differentiation genes would be expressed at late time points (D12, D18) Overall design: Total RNA obtained from murine TRP-1 transgenic Th17 cells activated with TRP-1 peptide. RNA was collected at several time points (Day 2, 4, 7, 12, 18) after activation and compared to unactivated, naïve CD4+ T cell controls.

体外肽特异性T细胞受体（TCR）激活后不同时间点的基因表达分析。本研究验证的核心假说为：激活相关基因将在早期时间点（D2、D4，即第2天、第4天）上调表达，而分化相关基因则会在晚期时间点（D12、D18，即第12天、第18天）特异性表达。实验设计概述：从经TRP-1肽激活的小鼠TRP-1转基因Th17细胞中提取总RNA，于激活后的多个时间点（第2、4、7、12、18天）收集RNA样本，并以未激活的初始CD4+ T细胞作为对照组进行比对分析。