SYP-4 phosphoproteomics characterization

The deletion of the last 114 amino acids of SYP-4 introduced severe defects in crossover regulation during meiosis I in C. elegans. Many processes during meiosis are regulated by post-translational modifications. Therefore, we aimed at identifying putative phosphorylation sites within the last 114 amino acids of SYP-4 by mass spectrometry to later test their functionality.

在秀丽隐杆线虫（Caenorhabditis elegans）中，删除SYP-4的最后114个氨基酸残基会引发减数分裂I期交换调控的严重缺陷。减数分裂过程中的诸多环节均受翻译后修饰调控。因此，本研究旨在通过质谱分析法鉴定SYP-4蛋白最后114个氨基酸区域内的潜在磷酸化位点，后续将对这些位点的功能进行验证。