Protection against autoimmunity is driven by thymic epithelial cell-mediated regulation of Treg development

Thymic education of Tregs is superior to negative selection in the prevention of autoimmunity. To address this hypothesis, TCR repertoires of thymic and peripheral Tcells (including Tregs) from conditional NIK KO and WT mice have been sequenced and compared. Overall design: Tregs cells have been sorted from spleen of 5 WT and 5 Foxn1CrexNIKflfl mice.

调节性T细胞（Tregs）的胸腺教育在自身免疫预防方面优于阴性选择。为验证该假说，研究人员对条件性NIK敲除（NIK KO）小鼠与野生型（WT）小鼠的胸腺及外周T细胞（含Tregs）的T细胞受体（TCR）库进行了测序与比较。总体实验设计：从5只野生型小鼠与5只Foxn1Cre×NIKfl/fl小鼠的脾脏中分选获得调节性T细胞。