Transcriptomic analysis of resistance and short-term induction response to pyrethroids, in Anopheles coluzzii legs

Here, we performed multiple transcriptomic analyses in An. coluzzii legs. First, we compared the leg vs whole body transcriptome to identify leg-specific genes. Next, we compared the leg transcriptomes of a multi-resistant strain (VK7) with two susceptible strains, to identify leg-expressed genes implicated in pyrethroid resistance. Last, to identify genes implicated in short-term deltamethrin induction, we investigated the leg transcriptome of VK7 after 1-hr deltamethrin exposure.

本研究对科卢兹按蚊（An. coluzzii）的足部开展了多项转录组分析。首先，我们比较了足部与全身体的转录组，以鉴定足部特异性基因；其次，将多抗品系（VK7）的足部转录组与两个敏感品系的足部转录组进行比对，以筛选出与拟除虫菊酯抗性相关的足部表达基因；最后，为鉴定与溴氰菊酯短期诱导相关的基因，我们对暴露于溴氰菊酯1小时后的VK7品系的足部转录组进行了分析。