Cydrasil-v3-reference-alignment.afa

This is a curated alignment generated using SSU-Align 0.1.1 with default parameters and masked using the ssu-mask feature of SSU-Align with per-alignment calculated masks. The alignment was then manually examined in Geneious version 8 to remove duplicate aligned sequences, non-16S rRNA sequences, and alignment errors. Sequences that were poorly aligned (typically due to the unlabeled inclusion of the ITS and/or 23S regions) were trimmed and removed if their length fell below the 1100 base pair threshold. The alignment was generated using the FASTA file cydrasil-v3-sequence-list.fasta found within the Cydrasil 3.0 figshare project. This alignment file is in aligned FASTA format.

本数据集为经人工精选的序列比对结果，采用SSU-Align 0.1.1版本及默认参数生成，并通过SSU-Align的ssu-mask功能，基于每一次比对独立计算得到的掩码完成序列屏蔽。随后，我们在Geneious 8版本软件中对该比对结果进行人工校验，以移除重复比对序列、非16S核糖体RNA（16S rRNA）序列以及比对错误。对于比对质量不佳（通常源于未标记纳入内转录间隔区（Internal Transcribed Spacer, ITS）和/或23S核糖体RNA（23S rRNA）区域）的序列，我们将其进行修剪处理，若修剪后长度低于1100碱基对阈值则予以移除。本比对基于Cydrasil 3.0的figshare共享项目中的FASTA格式文件cydrasil-v3-sequence-list.fasta生成，该比对文件采用比对版FASTA格式。