Figure S1 - Identification of B Cell Epitopes of Alcohol Dehydrogenase Allergen of Curvularia lunata

Sequence alignment of CADH with ADH from different sources. The sequences of alcohol dehydrogenase from various sources were aligned using Clustal W (1.81) multiple sequence alignment tool (http://align.genome.jp/) and manually optimized. The sequences are as follows: 1) Chain A of human alpha alcohol dehydrogenase, 2) Chain A, human beta-1 alcohol dehydrogenase, 3) Alcohol dehydrogenase E chain from Equus caballus (horse), 4) alcohol dehydrogenase from Rattus norvegicus (Norway rat), 5) Chain A of mouse alcohol dehydrogenase from Mus musculus, 6) alcohol dehydrogenase 1 from Zea mays, 7) alcohol dehydrogenase from Arabidopsis thaliana, 8) alcohol dehydrogenase II from Candida albicans, 9) alcohol dehydrogenase from Candida albicans, 10) alcohol dehydrogenase from Saccharomyces cerevisiae, 11) alcohol dehydrogenase from Cochliobolous lunatus, 12) alcohol dehydrogenase from Schizosaccharomyces pombe. Curvularia lunata ADH (CADH) accession number is highlighted in blue whereas the conserved residues are denoted by an asterisk (*). CADH sequence shows the presence of all 22 residues conserved across kingdoms as reported by Jornvall et al., 1987 (40). These residues are highlighted in yellow. (DOCX)

本数据集为不同来源乙醇脱氢酶（ADH，Alcohol Dehydrogenase）与弯孢菌乙醇脱氢酶（CADH，Curvularia lunata ADH）的多序列比对结果。本研究采用Clustal W（1.81版）多序列比对工具（http://align.genome.jp/）对各类来源的乙醇脱氢酶序列进行比对，并经人工优化调整。具体序列如下：
1. 人源α型乙醇脱氢酶A链；
2. 人源β-1型乙醇脱氢酶A链；
3. 马（Equus caballus）源乙醇脱氢酶E链；
4. 褐家鼠（Rattus norvegicus，又名挪威大鼠）源乙醇脱氢酶；
5. 小家鼠（Mus musculus，即实验小鼠）源乙醇脱氢酶A链；
6. 玉米（Zea mays）源乙醇脱氢酶1；
7. 拟南芥（Arabidopsis thaliana）源乙醇脱氢酶；
8. 白色念珠菌（Candida albicans）源乙醇脱氢酶II；
9. 白色念珠菌源乙醇脱氢酶；
10. 酿酒酵母（Saccharomyces cerevisiae）源乙醇脱氢酶；
11. 平脐蠕孢菌（Cochliobolous lunatus）源乙醇脱氢酶；
12. 粟酒裂殖酵母（Schizosaccharomyces pombe）源乙醇脱氢酶。
弯孢菌乙醇脱氢酶（CADH）的序列登录号以蓝色高亮标注，保守残基以星号（*）标记。如Jornvall等人1987年发表的研究（参考文献40）所述，CADH序列包含了跨生物界保守的全部22个残基，此类残基以黄色高亮标注。(DOCX)