Production of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography

We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.

本研究报道了一种快速高效的制备方法：以胰蛋白胨大豆肉汤（tryptic soy broth）为发酵培养基制备肺炎链球菌（Streptococcus pneumoniae）荚膜多糖，并采用固定化大豆凝集素作为亲和吸附剂对该产物进行纯化。原则上，通过选择适配的凝集素吸附剂，该策略可用于从其他链球菌血清型菌株中制备纯化的荚膜多糖。