Transcriptomic characterization of the impact of 2'-fucosyllactose supplementation on intestinal adaptation following ileocecal resection.

Purpose: To identify the impact of 2'-FL supplementation on adaptive response following extensive intestinal resection.Methods: Transcriptomic profiles were obtained from mice undergoing ileocecal recection (8-10 week old male mice) and again at 8 weeks post-surgery. At the time of resection and again at 8 weeks post-op, small bowel samples were obtained from treatment and control animals and submitted for mRNA profiling. During these 8 weeks treatment animals (n=3) received 2'-FL supplementationion while controls (n=3) received only standard diet.Results: We observe enrichment in genes and pathways related to anti-microbial peptides, metabolism, and energy processing. Supplementation of 2'-FL increases energy availability and enhances the adaptive response.

研究目的：明确2'-岩藻糖基乳糖（2'-FL）补充干预对广泛肠切除术后适应性应答的影响。
实验方法：选取8~10周龄雄性小鼠，分别在回盲肠切除术实施时及术后8周两个时间点采集样本，获取其转录组谱。于切除术操作时与术后8周，从干预组与对照组小鼠中采集小肠组织样本，用于mRNA转录组分析。干预组小鼠（n=3）在术后8周内接受2'-FL补充干预，对照组小鼠（n=3）仅喂食标准饲料。
实验结果：本研究观察到，与抗菌肽、代谢及能量代谢相关的基因与通路出现富集现象。2'-FL补充干预可提升机体能量可利用度，并强化术后适应性应答。