16S rDNA sequencing and mass spectrometry-based metabolite profiling of offspring in mice at 24 weeks old

Maternal overnutrition can dramatically increase the susceptibility of offspring to metabolic diseases,whereas maternal exercise may improve glucose metabolism in offspring. C57BL/6 female mice were randomly assigned to four subgroups according to a diet and exercise paradigm before and during pregnancy as follows:NC(fed with normal chow diet and sedentary),NCEx(fed with normal chow diet and running),HF (fed with high-fat diet and sedentary),and HFEx(fed with high-fat diet and running).Integrative 16S rDNA sequencing and mass spectrometry-based metabolite profiling were synchronously performed to characterize the effects of maternal exercise on the gut microbiota composition and metabolite alterations in offspring.

母体营养过剩可显著增加子代罹患代谢性疾病的易感性，而母体运动则可改善子代的葡萄糖代谢。本研究将C57BL/6雌性小鼠在孕前及孕期根据饮食与运动模式随机分为四个亚组，具体如下：NC组（普通饲料日粮+静坐饲养）、NCEx组（普通饲料日粮+跑轮运动）、HF组（高脂膳食+静坐饲养）以及HFEx组（高脂膳食+跑轮运动）。研究同步开展整合式16S核糖体DNA（16S rDNA）测序与基于质谱的代谢物谱分析，以解析母体运动对子代肠道菌群组成及代谢物变化的影响。